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目的:检测乳腺癌组织中死亡相关蛋白激酶(DAPK)、p53和Bcl-2表达,探讨DAPK、p53和Bcl-2在乳腺癌组织中的作用及其相互关系。方法:用免疫组化SP法检测42例乳腺增生组织和68例乳腺浸润性癌组织中DAPK、p53和Bcl-2的表达水平,分析它们与乳腺癌临床病理的关系。结果:p53蛋白在乳腺增生组织中的表达为阴性,在乳腺癌组织中阳性表达率为51.5%(35/68);p53的阳性率与乳腺癌的组织学分级、淋巴结转移呈正相关。乳腺癌组织中Bcl-2阳性表达率为69.1%(47/68),明显高于乳腺增生组织;Bcl-2的阳性率与乳腺癌的肿瘤大小、淋巴结转移呈负相关。DAPK在乳腺癌组织中的阳性表达率为42.6%(29/68),明显低于乳腺增生组织;DAPK的阳性率与乳腺癌的病理分型、组织学分级、淋巴结转移、p53及Bcl-2呈负相关。结论:DAPK、p53和Bcl-2蛋白可作为预测乳腺癌生物学行为的参考指标。DAPK可能抑制乳腺癌的侵袭转移,这个作用可能与p53、Bcl-2相关。
OBJECTIVE: To detect the expression of DAPK, p53 and Bcl-2 in breast cancer and to explore the roles and relationships of DAPK, p53 and Bcl-2 in breast cancer. Methods: The expressions of DAPK, p53 and Bcl-2 in 42 cases of breast hyperplasia and 68 cases of invasive breast cancer were detected by immunohistochemical SP method, and their relationship with the clinicopathology of breast cancer were analyzed. Results: The positive expression rate of p53 protein in breast hyperplasia tissue was 51.5% (35/68). The positive rate of p53 was positively correlated with the histological grade and lymph node metastasis of breast cancer. The positive rate of Bcl-2 in breast cancer tissues was 69.1% (47/68), which was significantly higher than that in breast hyperplasia tissues. The positive rate of Bcl-2 was negatively correlated with tumor size and lymph node metastasis in breast cancer. The positive rate of DAPK in breast cancer tissues was 42.6% (29/68), which was significantly lower than that in breast hyperplasia tissues. The positive rate of DAPK correlated with the pathological type, histological grade, lymph node metastasis, p53 and Bcl-2 Negative correlation. Conclusion: DAPK, p53 and Bcl-2 proteins can be used as predictors for the biological behavior of breast cancer. DAPK may inhibit the invasion and metastasis of breast cancer, this effect may be associated with p53, Bcl-2.