采用有机溶剂－水双液相体系作为反应介质，并用键合有—Ｃ６Ｈ５的无定型多孔硅胶作为分散剂增大两相间的接触面，用圆柱状假丝酵母脂肪酶对萘普生甲酯进行不对称水解．考察了酶浓度、体系ｐＨ值、温度、分散剂对反应的影响．当转化率为２４．３％时，产品萘普生的对映体过量值（ｅｅ）为９４．９％，剩余底物萘普生甲酯的对映体过量值（ｅｅ）为３０．５％，当以异辛烷作为有机相时，该体系酶催化水解的对映体比率（Ｅ）约为５０． An organic solvent-water two-liquid phase system was used as the reaction medium, and the interface between the two phases was enlarged by using amorphous porous silica bonded with -C6H5 as a dispersant. The naproxen methyl ester was treated with Candida cylindrica lipase Asymmetric hydrolysis. The effects of enzyme concentration, system pH, temperature and dispersant on the reaction were investigated. The enantiomeric excess (ee) of the product naproxen was 94.9% at a conversion of 24.3% and the enantiomeric excess (ee) of the remaining substrate naproxen was 30.5 %, When isooctane is used as the organic phase, the enantiomeric ratio (E) of enzymatic hydrolysis of this system is about 50%.