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本工作研究不同LET射线辐照对HepG2肝癌细胞辐射敏感性、周期进程和凋亡的影响,为重离子治疗癌症的临床应用积累基础数据。以0、0.5、1、2、4、8Gy剂量的12C6+离子及X射线分别照射处于指数生长期的HepG2细胞,用克隆形成率测定细胞辐射敏感性,通过流式细胞术测定细胞DNA含量以确定各时相细胞的比例及细胞凋亡情况。实验结果显示,12C6+离子辐照所致的HepG2细胞存活率明显低于X射线。随着吸收剂量的增加和修复时间的延长,12C6+离子能导致更显著的细胞S期阻滞、G2/M期阻滞延迟和细胞凋亡。说明与X射线相比,12C6+离子辐照能更有效地杀伤HepG2肝癌细胞并诱导其凋亡。
This work is to study the effects of different LET radiation on the radiosensitivity, cycle progression and apoptosis of HepG2 hepatocellular carcinoma cells and to accumulate the basic data for the clinical application of heavy ion therapy in cancer. HepG2 cells in exponential growth phase were irradiated with 12C6 + ions of 0, 0.5, 1, 2, 4, 8Gy doses and X-rays respectively. The cell radiation sensitivity was determined by the rate of colony formation and the DNA content of the cells was determined by flow cytometry to determine The proportion of cells in each phase and apoptosis. The experimental results showed that the survival rate of HepG2 cells induced by 12C6 + ion irradiation was significantly lower than that of X-ray. With the increase of absorbed dose and the prolongation of repair time, 12C6 + ion can lead to more significant S phase arrest, G2 / M arrest delay and apoptosis. It shows that 12C6 + ion irradiation can kill and induce HepG2 hepatoma cells more effectively than X-ray.