目的观察多路输液管路连接处的细菌污染情况和消毒效果。方法采用棉拭涂抹采样和细菌检测方法,检测多路输液管路连接处细菌污染和评价自制防护盒和酒精消毒效果。结果多路输液管路连接处细菌数在0~19 cfu/cm2,细菌污染超标率40%。使用自制防护盒和75%酒精局部消毒,局部存活菌数为0~1 cfu/cm2,消毒质量合格率为100%。结论多路输液管路连接处使用自制防护盒和75%酒精消毒,可避免细菌污染,预防感染的发生。 Objective To observe the bacterial contamination and the disinfection effect at the junction of multiple transfusion lines. Methods Cotton swab smear and bacterial detection methods were used to detect the bacterial contamination at the junction of multiple transfusion lines and evaluate the self-made protective case and alcohol disinfection effect. Results The number of bacteria in the junction of multiple transfusion lines ranged from 0 to 19 cfu / cm2, and the bacterial contamination exceeded the standard by 40%. Using homemade protective box and 75% alcohol local disinfection, the number of local viable bacteria is 0 ~ 1 cfu / cm2, disinfection quality pass rate of 100%. Conclusion Multiple infusion lines with self-made protective box junction and 75% alcohol disinfection can prevent bacterial contamination and prevent infection.