In this work, we report a comprehensive study about the eco-environmental effects of the shelter forest along the Tarim Desert Highway, including the effects on aeolian environment, soil, micro-climate, biodiversity, and groundwater. The results show that: (1) The movement of windblown sand near the ground surface was affected by the shelter forest. The wind speed and sediment transport rate in the shelter forest decreased by 64%―80% and 87.45%―99.02%, respectively. In addition, there were also significant changes in the sand flux structure, the sand grain size, and the deflation and deposition on the ground surface. (2) Compared to the natural mobile sand, the soil bulk density in the forest area decreased while the total salt content, the total porosity, and the water content increased. In addition, the soil fertility was significantly improved in the forest area, and showed the “first rapid, then slow” variation pattern. (3) The shelter forest showed positive effects on the micro-climate. Within the 6 m height above the ground, the air temperature in the shelter forest at different heights was lower than that in the mobile sand, while the air humidity was higher, while, the soil temperature was also lower in the shelter forest than mobile sand. (4) The number of soil microbial species increased significantly with the improvement of habitat in the shelterbelt. However, the population of different species was not distributed evenly across the surveyed area. (5) Currently, no significant effects of groundwater-pumping and forest-irrigation water have been found on the groundwater level and its salinity. The variation amplitude of both groundwater level and salinity was at the level of centimeters and 1g/L, respectively. No obvious variation trend has been observed. In this work, we report a comprehensive study about the eco-environmental effects of the shelter forest along the Tarim Desert Highway, including the effects on aeolian environment, soil, micro-climate, biodiversity, and groundwater. The movement of windblown sand near the ground surface was affected by the shelter forest. The wind speed and sediment transport rate in the shelter forest decreased by 64% -80% and 87.45% -99.02%, respectively. significant changes in the sand flux structure, the sand grain size, and the deflation and deposition on the ground surface. (2) Compared to natural sand sand, the soil bulk density in the forest area decreased while the total salt content, the total In addition, the soil fertility was significantly improved in the forest area, and showed the “first rapid, then slow ” variation pattern. (3) The shelter forest showed positive effects on the mi Within the 6m height above the ground, the air temperature in the shelter forest at different heights was lower than that in the mobile sand, while the air humidity was higher, while, the soil temperature was also lower in the shelter (4) The number of soil microbial species increased significantly with the improvement of habitat in the shelterbelt. However, the population of different species was not distributed evenly across the surveyed area. (5) Currently, no significant effects of groundwater-pumping and forest-irrigation water have been found on the groundwater level and its salinity. The variation amplitude of both groundwater level and salinity was at the level of centimeters and 1g / L, respectively. No obvious variation trend has been observed.