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目的 :分析我国呼吸道合胞病毒 (RSV)地方株SH蛋白基因的遗传特点、变异特征。方法 :从本室保存的不同年份、不同地区、不同流行特征的呼吸道合胞病毒地方株中抽取 7株 ,分别用RT PCR扩增其SH蛋白基因并克隆到PTZ18R载体中进行了序列测定和分析。结果 :我国RSV地方株间SH蛋白基因相比较变异的部位及形式很相似 ,地方株间核苷酸全序列的同源性为 97.0 %～ 10 0 %。结论 :RSV在我国不同地区的不同流行特征与SH蛋白基因的变异无明显关系
Objective: To analyze the genetic characteristics and variation of SH protein gene of respiratory syncytial virus (RSV) in China. Methods: Seven strains of local isolates of respiratory syncytial virus were collected from different years, regions and epidemics in our laboratory. The SH protein genes were amplified by RT-PCR and cloned into PTZ18R vector for sequencing and analysis . Results: The regions and forms of SH protein genes in RSV strains in China were similar. The homology of nucleotide sequences in local strains was 97.0% ~ 100%. Conclusion: There is no significant relationship between the different epidemiological characteristics of RSV and the variation of SH protein in different regions of China