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目的探讨CD4+CD25+调节性T细胞(Treg)在稽留流产患者外周血中的表达情况。方法 30例稽留流产患者作为观察组,30例自愿进行人工流产的健康早孕者作为对照组,利用流式细胞分析技术检测两组研究对象外周血中CD4+CD25+Treg表达情况,计算得出CD4+CD25+Treg细胞占T淋巴细胞的比例,并进行组间比较。结果对照组CD4+CD25+Treg细胞占T淋巴细胞的比例为(5.31±2.61)%,观察组CD4+CD25+Treg细胞占T淋巴细胞的比例为(4.07±1.67)%,观察组CD4+CD25+Treg细胞占T淋巴细胞的比例明显低于对照组,差异具有统计学意义(P<0.05)。结论 CD4+CD25+Treg降低可能参与了稽留流产发生过程,而其水平在妊娠早期表达明显升高,可能是与正常妊娠的维持以及母胎免疫耐受调节等有关。
Objective To investigate the expression of CD4 + CD25 + regulatory T cells (Tregs) in peripheral blood of missed abortion patients. Methods 30 cases of missed abortion patients as observation group, 30 cases of voluntary pregnancy induced abortion as a control group, the use of flow cytometry analysis of peripheral blood CD4 + CD25 + Treg expression in two groups were calculated CD4 + Ratio of CD25 + Treg cells to T lymphocytes and compared between groups. Results The proportion of CD4 + CD25 + Treg cells to T lymphocytes in the control group was (5.31 ± 2.61)%, the percentage of CD4 + CD25 + Treg cells in T cells in the observation group was (4.07 ± 1.67)%, and the percentage of CD4 + CD25 + Treg cells in T lymphocytes was significantly lower than the control group, the difference was statistically significant (P <0.05). Conclusions The decrease of CD4 + CD25 + Treg may be involved in the process of missed abortion. However, the level of CD4 + CD25 + Treg may be related to the maintenance of normal pregnancy and the regulation of immune tolerance of maternal fetus.