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研制了一款环介导等温扩增芯片,开发了相应的检测控制系统,并在此芯片上进行了金葡菌环介导等温扩增反应最佳扩增条件的研究.实现了在聚碳酸酯芯片上进行环介导等温扩增实验,与传统管式聚合酶链式反应相比,它需要的样品和试剂量更少.通过在不同温度下进行实验,获得了在芯片微体系条件下,进行环介导等温扩增反应检测金葡菌的最佳温度条件为61℃,提高了环介导等温扩增芯片的检测速度,缩短了核酸分析时间.
A ring-mediated isothermal amplification chip was developed and a corresponding detection and control system was developed, and the optimal amplification conditions for S. aureus ring-mediated isothermal amplification reaction were studied on this chip. Loop-mediated isothermal amplification experiments on ester chips, which required fewer samples and reagents compared to the traditional tube-type PCR.The experiments were carried out at different temperatures, , The optimal conditions for detecting S. aureus by ring-mediated isothermal amplification reaction were 61 ℃, which improved the detection speed of ring-mediated isothermal amplification chip and shorten the time of nucleic acid analysis.