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用注射接种法测定了水稻白叶枯病菌诱导烟草过敏性反应的能力,发现所有测试菌株在诱导烟草过敏性反应出现时间和组织中的菌量方面都有差异。不同来源和小种的原始菌株都具有诱导能力,转座子Tn5诱变的突变体菌株只有一个突变体 XcoM3105失去了诱导能力.亲本菌株在烟草叶片组织中的种群量迅速下降,细菌被限制在接种区内,突变体菌株在组织中可以繁殖和扩展。测定细菌细胞不同组分的诱导活性,发现脂多糖是诱导烟草过敏性反应的信号物质,诱导活性中心是多糖体组分.亲本菌株和突变体的脂多糖和多糖体表现相同的诱导活性。对这一结果进行了讨论.
Inoculation assay was used to determine the ability of Xanthomonas oryzae to induce tobacco hypersensitive response. All the tested strains were found to be different in inducing the onset of tobacco hypersensitivity reaction and the amount of bacteria in the tissue. The original strains of different origins and races all had the ability of inducing, and only one mutant XcoM3105 mutants of the transposon Tn5 mutants lost the ability to induce.The population of the parental strain in tobacco leaf tissue decreased rapidly, and the bacteria were limited to Within the inoculation zone, the mutant strain can multiply and expand in the tissue. The inducing activity of different components of bacterial cells was determined and it was found that lipopolysaccharide was a signal substance inducing the hypersensitive reaction of tobacco and the active center was polysaccharide component.The lipopolysaccharide and polysaccharide of the parent strain and mutant showed the same inducing activity. This result is discussed.