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目的观察碳酸氢钠对旋毛虫幼虫感染性及生殖力的影响。方法将旋毛虫肌幼虫在体外经1%碳酸氢钠处理不同时间后观察其活力。100只雄性昆明小鼠随机分成10组(每组10只),1～3组中每组5只分别喂饲经碳酸氢钠或生理盐水浸泡不同时间(24、36、48h)含300条旋毛虫肌幼虫的小鼠肌肉(约0.02g);4～9组分别用不同剂量碳酸氢钠或食用醋(总酸浓度4.5%,pH3.05)灌胃后0.5h喂饲含300条肌幼虫肉块,10组为灌服生理盐水对照组。于喂饲后第7天和第42天每组剖杀5只小鼠,分别观察肠道成虫数、肌幼虫数及生殖力指数(RCI)。结果肌幼虫经碳酸氢钠处理24～240h的死亡率与生理盐水对照组相比差异无统计学意义(P>0.05)。小鼠喂饲经碳酸氢钠处理24、36h和48h旋毛虫肌幼虫后42d,其肌幼虫数(84467、84600条和82300条)均明显高于生理盐水对照组(12650、6460条和1240条)(P<0.05)。灌饲0.4、0.6ml碳酸氢钠小鼠的肠道成虫数(193、203条)与RCI(323.77、326.55)均明显高于食醋对照组(68、67条;217.55、195.33)及生理盐水对照组(145条,268.13)(P<0.05)。结论碳酸氢钠可明显增加旋毛虫的感染性与生殖力。
Objective To observe the effects of sodium bicarbonate on the infectivity and fecundity of Trichinella spiralis larvae. Methods Trichinella spiralis muscle larvae were treated with 1% sodium bicarbonate in vitro at different times and observed their vitality. 100 male Kunming mice were randomly divided into 10 groups (10 rats in each group), 5 rats in each group from 1 to 3 were fed with sodium bicarbonate or normal saline for different time (24, 36, 48 hours) Caterpillar muscle larvae mouse muscle (about 0.02g); 4 to 9 groups were fed with different doses of sodium bicarbonate or edible vinegar (total acid concentration 4.5%, pH3.05) 0.5h after feeding containing 300 muscle larvae Meat, 10 groups for the saline control group. Five mice were killed in each group on the 7th day and the 42nd day after feeding, and the numbers of adult worms, muscle larvae and fecundity index (RCI) were observed. Results The mortality of muscle larvae treated with sodium bicarbonate for 24 ~ 240 h was not significantly different from that of saline control group (P> 0.05). The number of muscle larvae (84467, 84600 and 82 323) in mice fed with sodium bicarbonate for 24, 36 and 48 h after treatment was significantly higher than that of the saline control group (12650, 6460 and 1240 ) (P <0.05). The number of intestinal tract adults (193,203) and RCI (323.77,326.55) fed 0.4,0.6 ml sodium bicarbonate mice were significantly higher than those of the vinegar control group (68,67; 217.55,195.33) and saline Control group (145, 268.13) (P <0.05). Conclusion Sodium bicarbonate can significantly increase Trichinella spiralis infectivity and fecundity.