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目的 用重组日本血吸虫 2 2 k D表膜蛋白 (r Sj2 2 )免疫水牛 ,检测特异性 Ig G抗体的应答水平 ,并观察抗血吸虫的保护效果。方法 用抗原 (r Sj2 2 )加佐剂 (Quil- A)肌肉注射免疫水牛 ,以10 0 0条日本血吸虫尾蚴攻击感染 ,感染后 5 5 d剖杀 ,计算减虫和减卵率。用免疫印斑和 EL ISA方法测定抗体反应。结果 免疫组每头牛血清均能特异地识别 r Sj2 2 k D抗原和成虫抗原中的 2 2 k D分子 ,特异性 Ig G抗体滴度达 1:2 5 6 0 0。与 Quil- A对照组相比 ,减虫率仅 8.5 % ,肝卵 EPG减少12 .3% ,粪卵 EPG减少 2 6 .8% ,但均无统计学意义。结论 用 r Sj2 2 k D抗原免疫水牛诱导的特异性Ig G抗体不能发挥免疫保护作用
OBJECTIVE: To immunize buffaloes with recombinant Schistosoma japonicum 2 2 kD membrane protein (r Sj2 2) to detect the response of specific Ig G antibodies and to observe the protective effect of the anti-schistosomes. Methods Buffaloes were injected intramuscularly with antigens (rSj2 2) and adjuvant (Quil-A), and infected with 100 schistosoma japonicum cercariae. Antibody responses were determined by immunoblotting and ELISA. Results Each bovine serum in the immunized group could specifically recognize 22 kD molecules of rSj2 2 kD antigen and adult antigen, and the titer of specific Ig G antibody was 1: 2560. Compared with Quil-A control group, worm reduction rate was only 8.5%, liver egg EPG decreased 12.3%, feces egg EPG decreased 26.8%, but no statistically significant. Conclusion Specific Ig G antibodies induced by buffalo immunization with r Sj2 2 k D antigen do not exert an immunoprotective effect