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目的：观察葡萄球菌肠毒素ASEA对Hep－2细胞株诱导凋亡机制。方法：应用MTT比色法和流式细胞术检测SEA对Hep－2细胞增殖抑制率和对细胞增殖周期的影响,同时应用同位素法检测Hep－2细胞内CAMP水平。结果：SEA对Hep－2细胞株有明显的抑制作用,且呈剂量依赖性。流式细胞术分析,SEA能阻止G1期细胞向S期的过程,G1期细胞堆积。同位素分析,Hep－2细胞内CAMP水平下降。结论：SEA能诱导Hep－2细胞凋亡,抑制Hep－2细胞增殖,具有细胞毒性效应。
Objective: To observe the mechanism of apoptosis induced by Staphylococcal enterotoxin ASEA on Hep-2 cell line. Methods: MTT colorimetric assay and flow cytometry were used to detect the effect of SEA on Hep-2 cell proliferation inhibition rate and cell proliferation cycle. Simultaneously, the level of CAMP in Hep-2 cells was detected by isotopic method. RESULTS: SEA inhibited the Hep-2 cell line in a dose-dependent manner. Flow cytometry analysis showed that SEA can prevent G1 phase cells from going to S phase and G1 phase cells accumulate. Isotope analysis showed that the level of CAMP in Hep-2 cells decreased. Conclusion: SEA can induce apoptosis of Hep-2 cells and inhibit the proliferation of Hep-2 cells with cytotoxicity.