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本工作采用细胞离休孵育方法观察了抑制素α亚基Ｎ末端片段Ｐ~（３２）（１－３２）、Ｐ~（３２－Ｔｙｒ）（１－３２－Ｔｙｒ）和外源性单胺能神经递质─儿茶酚胺［去甲肾上腺素（ＮＥ），肾上腺素（Ｅ），多巴胺（ＤＡ）和五羟色胺（５－ＨＴ）］对大鼠黄体孕酮分泌的影响。结果显示：（１）Ｐ~（３２）和Ｐ~（３２－Ｔｙｒ）１００ｎｇ显著抑制黄体细胞的孕酮（Ｐ_４）分泌。（２）儿茶酚胺－肾上腺素和去甲肾上腺素（０．１ｍｍｏｌ／Ｌ），以及ＤＡ（１０μｍｏｌ／Ｌ）均使黄体基础与ｈＣＧ致Ｐ_４分泌明显增加（Ｐ＜０．００１）。对基础Ｐ_４分泌，它们的强度是ＮＥ＞Ｅ＞ＤＡ，而对ｈＣＧ致Ｐ_４分泌则相反，ＤＡ＞Ｅ＞ＮＥ。Ｐ~（３２），Ｐ~（３２－Ｔｙｒ）对ＮＥ刺激的基础和ｈＣＧ致Ｐ４分泌有所加强，而对Ｅ刺激的Ｐ_４分泌则显著抑制。（３）酚妥拉明（α）与心得安（β）阻断剂本身可显著降低基础与ｈＣＧ致Ｐ_４分泌，心得安对ｈＣＧ作用的抑制明显大于酚妥拉明；两者均加强Ｐ~（３２），Ｐ~（３２－Ｔｙｒ）对Ｐ_４分泌的抑制。（４）５－ＨＴ０．５μｍｏｌ／Ｌ则显著抑制黄体Ｐ_４分泌，并使Ｐ~（３２），Ｐ~（３２－Ｔｙｒ）的抑制作用大为减弱。以上结果提示：（１）黄体细胞上?
In this work, we observed the inhibitory effects of inhibin α-subunit N-terminal fragment of P322 (1-32), P32-Tyr (1-32-Tyr) and exogenous monoaminergic Effects of neurotransmitters-catecholamines [norepinephrine (NE), epinephrine (E), dopamine (DA) and serotonin (5-HT)] on progesterone secretion in rats. The results showed that: (1) P ~ (32) and P ~ (32-Tyr) 100ng significantly inhibited progesterone (P_4) secretion of luteal cells. (2) Both catecholamine-epinephrine and norepinephrine (0.1 mmol / L) and DA (10 μmol / L) increased the secretion of P 4 in corpus luteum and hCG significantly (P <0.001). The basal P_4 secretion, their intensity is NE> E> DA, while the secretion of hCG induced P_4 contrary, DA> E> NE. P32 and P32-Tyr enhanced the secretion of P4 and the secretion of P4 of hCG, but inhibited the secretion of P_4. (3) Phentolamine (α) and propranolol (β) blockers can significantly reduce the basal and hCG-induced P 4 secretion, propranolol inhibited the hCG effect significantly more than phentolamine; both enhanced P ~ (32), P ~ (32-Tyr) inhibition of P 4 secretion. (4) 5-HT0.5μmol / L significantly inhibited the secretion of P_4 in corpus luteum, and greatly reduced the inhibitory effect of P ~ (32) and P ~ (32-Tyr). The above results suggest: (1) luteal cells?