采用两根反相柱串联的多维HPLC,直接进样测定血浆/尿中抗癌药物六亚甲基二乙酰胺(HMBA)。第一根柱使用胶束流动相,作为样品的纯化和富集步骤,第二根柱为分析柱。紫外检测波长为210 nm。药物的回收率在94.0～100.9%之间,相对标准偏差为1.88%。用该法对十二位不同恶性肿瘤患者的282个血浆/尿样品进行了测定,得到了满意的结果并建立了药代动力学曲线。 Multi-dimensional HPLC with two reversed-phase columns in series was used for direct injection to determine plasma / urinary anticancer drug hexamethylenedi-acetamide (HMBA). The first column uses a micellar mobile phase as a purification and enrichment step for the sample, with the second column being the analytical column. UV detection wavelength of 210 nm. Drug recovery was 94.0 ~ 100.9%, the relative standard deviation of 1.88%. Using this method, we measured 282 plasma / urine samples from 12 patients with different malignancies and obtained satisfactory results and established pharmacokinetic curves.