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本文报道,几种常见的解热止痛药与可待因等共存时的高压液相层析测定法。层析条件:层析柱μBon-dapak C_(18),30cm×4mm(内径),流动相含有19%甲醇的M/100磷酸二氢钾液,并用磷酸调节pH为2.3,进样量20μl,内含阿司匹林2.0μg、咖啡因0.5μg、其余样品均为1.0μg,流速2ml/分,用可变波长紫外分光检测器,以峰高法计算含量。线性范围均为1.25～3.0μg。除扑热息痛外,供试溶液的制备均可使用乙醇,唯阿司匹林应注意新鲜制备。三种市售处方与自配4种组分的混合物都能得到很好的结果。由于市售商品不可能同时具备上述七种组分,因之如选择适当的内标也是容易的。作者还试用过未经磷酸调节pH的流动相。降低流动相的pH值,弱酸性组分滞留时间增加,弱碱性组分滞
This article reports, several common antipyretic analgesics and codeine coexistence of high pressure liquid chromatography assay. Chromatographic conditions: column μBon-dapak C_ (18), 30cm × 4mm (ID), the mobile phase containing 19% methanol M / 100 of potassium dihydrogen phosphate and phosphoric acid to adjust the pH to 2.3, the injection volume 20μl, Containing aspirin 2.0μg, caffeine 0.5μg, the remaining samples were 1.0μg, flow rate 2ml / min, with variable wavelength UV spectrophotometer to calculate the peak height method. Linear range of 1.25 ~ 3.0μg. In addition to paracetamol, the test solution can be prepared using ethanol, aspirin should pay attention to fresh preparation. Three commercially available formulations with self-formulated blends of four components yielded good results. Since it is not possible for commercially available products to have the above seven components at the same time, it is also easy to select an appropriate internal standard. The authors have also tried the pH-free mobile phase without phosphoric acid. Reduce the pH of the mobile phase, weak acid component retention time increases, weak alkaline components stagnation