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本文用使君子酸(quisqualic acid,QA)分两点注入Sprague-Dawley大鼠左侧基底大细胞核制成Alzheimer病动物模型。破坏1周后,一组动物将同种胚基底前脑悬液注入模型鼠的额叶和顶叶,另一组动物则将胚基底前脑悬液原点注入破坏区。移植后5个月行为测试被动回避试验及主动回避试验,与破坏后1周的测试结果相比有显著性差异(p<0.05),表明移植后行为有明显改善。但两组动物移植后行为改善的程度无明显差异。形态学观察发现两组动物移植区均有AChE阳性神经元及阳性纤维终末形成的斑片。原位移植区范围较皮质移植区略大,移植区所见AChE阳性神经元形态与正常基底大细胞核的细胞相似。实验结果表明原位移植亦能获得与靶区移植同样的效应,提示基底大细胞核区在受损后可能产生某种神经营养因子,促进了胚脑细胞的存活。Alzheimer病模型鼠机能的恢复不一定与神经网络的重建有关,而与具有产生ACh递质的内源性微泵有关。
In this paper, Alzheimer’s disease animal model was established by injecting quisqualic acid (QA) into the left basal cell nucleus of Sprague-Dawley rats. After one week of vandalism, one group of animals injected the same embryonic basal forebrain suspension into the frontal lobe and parietal lobe of the model mouse, while the other group injected the origin of the embryo basal forebrain suspension into the damaged area. Five months after transplantation, the passive avoidance test and the passive avoidance test showed significant differences (p <0.05) compared with the results of 1 week after the transplantation, which indicated that the behaviors of the patients after transplantation were significantly improved. However, there was no significant difference in the behavioral improvement between the two groups after transplantation. Morphological observation showed that AChE-positive neurons and positive fibrotic patches were formed in both groups. The size of the orthotopic transplantation zone was slightly larger than that of the cortical transplantation zone. The morphology of AChE positive neurons in the transplantation zone was similar to that of the normal basal cell nucleus. The experimental results show that orthotopic transplantation can also achieve the same effect as target transplantation, suggesting that some large neuronal nutrition factors may be produced in the basal macrophage nucleus and promote the survival of embryonic brain cells. Restoration of Alzheimer’s disease model rats is not necessarily related to neural network reconstruction but to endogenous micropumps that produce ACh neurotransmitters.