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在骨巨细胞三种类型细胞的性质和组织发生相互关系的研究方面。酸性磷酸酶超微结构定位是很重要方法之一。但国内有关骨巨细胞瘤酸性磷酸酶超微结构定位未曾见有报道。我们对五例骨巨细胞瘤,大白鼠肝脏及小白鼠肝脏进行了酸性磷酸酶电镜定位;同时,用Gomnri氏硫化铅法的酸性磷酸酶组织化学方法进行观察作为比较。本文报告戊二醛固定液对显示酸性磷酸酶活性的影响,提出固定时间不得超过24小时,并应置于4℃冰箱。此外对trise/maleate缓冲及醋酸缓冲液进行比较,发现在骨巨细胞瘤,大白鼠及小白鼠肝脏电镜定位醋酸缓冲液比较好。在观察酸性磷酸酶超微结构时,超薄切片可不经过柠檬酸铅染色而直接观察,比经过柠檬酸铅染色,结构更为清晰。
In the study of the relationship between the nature and organization of three types of giant cell bone cells. Ultrastructural localization of acid phosphatase is one of the most important methods. However, there is no report about the ultrastructural localization of acid phosphatase in giant cell tumor of bone in China. We performed acid phosphatase microscopy on five cases of giant cell tumor of bone, rat liver and mouse liver, and compared them with acid phosphatase histochemistry using Gomnri’s lead sulphate method. This article reports glutaraldehyde fixative on the display of acid phosphatase activity, proposed fixed time shall not exceed 24 hours, and should be placed in 4 ℃ refrigerator. In addition to the trise / maleate buffer and acetate buffer were compared, found in giant cell tumor of bone, white rat and liver electron microscopy acetate acetate buffer is better. In observing the ultrastructure of acid phosphatase, ultrathin sections can be directly observed without lead citrate staining and stained more clearly than lead citrate.