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目的 探讨子宫颈癌中高危型人乳头状瘤病毒(high-risk humanpapilloma virus,HR-HPV)感染与c-jun和c-fos蛋白表达的关系及临床意义。方法 采用Cervista技术对70例子宫颈鳞状细胞癌(cervical squmaous cell carcinoma,CSCC)、60例子宫颈上皮内病变(cervical intraepithelial neoplasia,CIN)及20例慢性子宫颈炎行HR-HPV检测,同时应用免疫组化SP法检测AP-1通路重要组份c-jun和c-fos蛋白的表达,分析HR-HPV感染与c-jun和c-fos蛋白表达的相关性及与子宫颈癌临床病理特征的关系。结果 70例CSCC中69例HR-HPV呈阳性,阳性率98.6%(69/70),其中A9组阳性占比最高,达85.5%(59/69)。70例CSCC中c-jun和c-fos的阳性率分别为80%(56/70)、85.7%(60/70),30例CIN2+CIN3中的阳性率均为70%(21/30),30例CIN1中的阳性率分别为20%(6/30)、23.3%(7/30),20例慢性子宫颈炎中的阳性率均为0。CSCC组与CIN组及慢性子宫颈炎组相比差异有统计学意义(P<0.05)。c-jun、c-fos阳性率在CSCC的临床分期、组织学分级等分组中差异有统计学意义(P<0.05);CSCC中HR-HPV阳性与c-jun、c-fos的表达均呈正相关(P<0.01)。结论 CSCC中HR-HPV感染具有显著的亚型特点,且与c-jun和c-fos表达呈正相关,提示HR-HPV感染后AP-1通路活化可能与肿瘤的发生、发展相关。
Objective To investigate the relationship between the expression of c-jun and c-fos in high-risk human papilloma virus (HR-HPV) and its clinical significance in cervical cancer. Methods Cervista technique was used to detect HR-HPV in 70 cases of cervical squamous cell carcinoma (CSCC), 60 cases of cervical intraepithelial neoplasia (CIN) and 20 cases of chronic cervicitis. At the same time, The expression of c-jun and c-fos, an important component of AP-1 pathway, was detected by SP method. The correlation between HR-HPV infection and the expression of c-jun and c-fos protein and the clinicopathological characteristics of cervical cancer . Results The positive rate of HR-HPV in 69 CSCC cases was 98.6% (69/70). The positive rate of A9 group was 85.5% (59/69). The positive rates of c-jun and c-fos in 70 cases of CSCC were 80% (56/70) and 85.7% (60/70), respectively. The positive rates of CIN2 and C-fos in 70 cases were 70% (21/30) . The positive rates of CIN1 in 30 cases were 20% (6/30) and 23.3% (7/30), respectively. The positive rates of all 20 cases were 0 in chronic cervicitis. There was significant difference between CSCC group and CIN group and chronic cervicitis group (P <0.05). The positive rates of c-jun and c-fos in CSCC were significantly different between the clinical stage and histological grade (P <0.05). The positive rates of c-jun and c-fos in CSCC were positive Related (P <0.01). Conclusion HR-HPV infection in CSCC has significant subtypes and is positively correlated with the expression of c-jun and c-fos, suggesting that the activation of AP-1 pathway may be related to the occurrence and development of tumor after HR-HPV infection.