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随着毒品滥用现象的蔓延,提供简便、经济、有效的毒品检测技术显得日益重要。本文采用本实验室改良的头发消化技术和RIA对122例海洛因滥用者及30名正常人头发吗啡含量进行检测分析,现报告如下。 方法和对象 一、方法: 头发的消化法:取被检者头发0.5g置于50ml梨形瓶内,加入0.3N NaOH溶液10ml煮沸15分钟,用9N HCl溶液调pH为7.0～7.2以3000rpm分~(-1)的速度离心5分钟,消化均匀,未见颗粒呈半透明状液体,取样100μl作RIA ~(125)Ⅰ-吗啡放免试剂盒由海军总医院提供,按说明书操作,用HH600型γ放免分析仪检测。
With the spread of drug abuse, it has become increasingly important to provide simple, economical and effective drug detection technologies. In this paper, the laboratory modified hair digestion technology and RIA on 122 cases of abuse of heroin and 30 normal people hair morphine content analysis and testing are as follows. Method and object A method: hair digestion: The subjects were seized hair 0.5g 50ml pear-shaped bottle, add 0.3N NaOH solution 10ml boiled for 15 minutes, with 9N HCl solution adjusted to pH 7.0-7.2 3000rpm points Centrifugation for 5 minutes at ~ (-1), digestion, no particles were translucent liquid sampling 100μl for RIA 125I Ⅰ-morphine radioimmunoassay kit provided by the Navy General Hospital, according to instructions, with HH600 type γ RIA analyzer test.