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水稻叶鞘、茎节、根尖、幼穗、未成熟胚、成熟胚等不同外植体的离体培养,均已获得了再生植株。自1987年起,作者就水稻不同基因型的三种外植体,在相同培养条件下进行离体培养,以探索不同基因型,不同外值体的愈伤组织诱导率及植株再生状况。材料与方法以花寒早、SH_(33)(花培育种后代)、城特_(232)、寒丰、桂农_(12)(杂交品种)、太湖青、铁桂丰、铁杆老来青(地方品种)等八个粳稻品种作为供试材料。幼穗、叶鞘、茎节等外植体取自大田生长的植株。将剑叶叶鞘与倒二叶叶枕距为3～5cm的植株从基部切取,剪去叶片,在无菌条件下用70％的乙醇逐层表面消毒并剥去叶鞘,将最里面的叶鞘或幼穗、茎组织切开,每一叶鞘切成6块,每一幼穗切成1mm左
Regeneration plants have been obtained from in vitro culture of rice leaf sheaths, stem nodes, apices, spikelets, immature embryos and mature embryos. Since 1987, the authors conducted in vitro culture of three explants with different genotypes under the same culture conditions to explore the callus induction rate and plant regeneration status of different genotypes and different outer values. MATERIALS AND METHODS: The experiment was carried out with Hu Han-han, SH_ (33) (the offspring of flower cultivars), Sitai_ (232), Hanfeng, Guinong_ (12) (hybrids), Taihuqing, Tieguifeng, Qing (local varieties) and other eight varieties of japonica rice as the test material. Young spikes, leaf sheaths, stem nodes and other explants were taken from plants grown in the field. The leaves of the flag leaf sheath and the next two leaf pillow spacing of 3 ~ 5cm plant cut from the base, cut the leaves, sterile conditions with 70% ethanol layer surface disinfection and stripping the leaf sheath, the innermost leaf sheath or Spikelets and stems were dissected, each leaf sheath cut into 6 pieces, each panicle cut into 1mm left