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玉米幼叶愈伤组织的形成能力因其形态学部位的不同而呈显著差异 ,从基部向上依次递减 ,幼叶外植体的内源 IAA和 ABA水平亦相应地呈递减趋势。 2 ,4 - D及与 BA组合对基部切段的愈伤组织形成无显著影响 ,愈伤组织生长符合 L ogistic曲线 ,单独使用 BA不利于愈伤组织形成 ;采用 2 mg/L 2 ,4 - D与 0 .5mg/L BA的组合最有利于胚性愈伤组织的诱导。培养物内源 IAA水平随培养时间显著下降 ;2 ,4 - D可进一步降低 IAA水平 ,但显著提高内源 ABA水平 ,2 mg/L 2 ,4 - D作用最为显著。伴随着胚性愈伤组织的发生 ,内源 ABA相应地出现一个峰值。玉米胚性发生能力的大小与培养物内源 ABA水平的高低密切相关 ,2 ,4 - D可能是决定 ABA水平的因素之一。
The formation ability of callus in maize leaves was significantly different due to their different morphological locations, decreasing in descending order from the base. The levels of endogenous IAA and ABA in young leaves explants decreased accordingly. 2, 4 - D and BA had no significant effect on the callus formation of basal segment, callus growth accorded with L ogistic curve, BA was not conducive to callus formation when used alone; with 2 mg / L 2, 4 - The combination of D with 0 .5 mg / L BA most favored the induction of embryogenic callus. The level of endogenous IAA in culture decreased significantly with the incubation time. 2,4 and 4 - D reduced the level of IAA, but significantly increased the levels of endogenous ABA. The effect of 2 mg / L 2 and 4 - D was the most significant. Accompanied by the occurrence of embryogenic callus, endogenous ABA corresponding to a peak. The size of embryogenesis ability of maize is closely related to the level of endogenous ABA in culture. 2,4 - D may be one of the factors determining ABA level.