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制备了基于L-半胱氨酸、纳米金自组装的新型葡萄糖生物传感器。用L-半胱氨酸修饰裸金电极,并利用静电吸附的原理固定纳米金,最后通过交联剂使酶固定在修饰后的电极上制成葡萄糖生物传感器。L-半胱氨酸-纳米金以及酶的修饰提高了电极传递电子的能力。通过正交试验选取了最佳的交联条件:6 g/L葡萄糖氧化酶、0.6 g/L过氧化氢酶、0.03%1-乙基-[3-二甲基氨基丙基]碳酰二亚胺盐酸化物、0.03%N-羟基琥珀酰亚胺及p H 7.0的缓冲溶液。该传感器对葡萄糖溶液浓度有良好的线性关系,线性范围为1~16 mmol/L,线性回归方程为I=0.2512c+1.9583,相关系数达到0.9996,具有一定的抗干扰能力。该葡萄糖生物传感器电流大小与葡萄糖浓度呈现良好的线性关系,在临床检验上具有一定的应用前景。
A novel glucose biosensor based on L-cysteine and gold nanoparticles was prepared. L-cysteine is used to modify the bare gold electrode, and the principle of electrostatic adsorption is used to fix the nano gold. Finally, the enzyme is immobilized on the modified electrode by the crosslinking agent to make a glucose biosensor. The modification of L-cysteine-gold nanoparticles and enzymes increases the electrode’s ability to deliver electrons. The optimum cross-linking conditions were selected by orthogonal test: 6 g / L glucose oxidase, 0.6 g / L catalase and 0.03% 1-ethyl- [3-dimethylaminopropyl] Imine hydrochloride, 0.03% N-hydroxysuccinimide and a buffer solution of pH 7.0. The sensor has a good linear relationship with the concentration of glucose solution, the linear range of 1 ~ 16 mmol / L, linear regression equation I = 0.2512c + 1.9583, the correlation coefficient of 0.9996, with a certain anti-interference ability. The glucose biosensor current size and glucose concentration showed a good linear relationship in the clinical examination has a certain application prospects.