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6∶2氟调醇(6∶2 FTOH)是一种多氟烷基物质,近年被广泛用于工业和消费品中,对环境有潜在威胁,但目前关于6∶2FTOH及其降解产物对沉积物中微生物群落结构的影响还不清楚.本研究的目的是通过基因分析方法探索6∶2 FTOH生物降解对表层沉积物中细菌群落结构的影响.从天津海河采集表层沉积物和河水,在实验室进行微宇宙实验,通过LC-MS/MS测定6∶2 FTOH及其降解产物的浓度,通过变性梯度凝胶电泳和高通量测序分析细菌的群落结构.结果表明,6∶2 FTOH在微生物的作用下可发生降解(半衰期小于3 d),生成6∶2 FTCA、6∶2 FTUCA等中间产物和5∶2 FT Ketone、5∶2 s FTOH、PFHx A、PFPe A、PFBA、5∶3 Acid等稳定产物,该过程对沉积物细菌群落结构产生明显影响,引起细菌群落丰富度和多样性的变化.在6∶2 FTOH降解的不同阶段,细菌的变化和优势菌群略有不同.根据100 d的实验结果,从门的分类水平看,6∶2 FTOH生物降解引起绿弯菌门丰度大幅上升(+24.8%)、变形菌门和厚壁菌门丰度大幅下降(-17.8%和-15.9%).从纲的分类水平看,6∶2 FTOH生物降解引起丰度上升较大的有厌氧绳菌纲(+19.6%)和δ-变形菌纲(+4.3%),引起丰度下降较大的有ε-变形菌纲(-20.0%)、梭菌纲(-10.1%)、芽孢杆菌纲(-5.8%)和γ-变形菌纲(-4.2%).从属的分类水平看,6∶2 FTOH生物降解引起丰度上升较大的有Anaerolineaceae_uncultured(+19.1%)和硫碱球菌属(+13.3%),引起丰度下降较大的有弧菌属(-14.1%)、硫单胞菌属(-13.2%)、芽孢杆菌属(-5.1%)、Sulfurovum(-4.2%)和Fusibacter(-4.1%).这些结果有助于预测环境中细菌对多氟烷基物质污染的响应及筛选可降解多氟烷基物质的细菌.
6: 2 Fluorolcohol (6: 2 FTOH) is a polyfluoroalkyl material that has been widely used in industrial and consumer products in recent years and poses a potential threat to the environment. However, the current research on the effect of 6: 2 FTOH and its degradation products on sediment The purpose of this study is to explore the effect of 6: 2 FTOH biodegradation on bacterial community structure in surface sediments by genetic analysis.Cement sediments and river water are collected from Haihe River in Tianjin, Micro-universe experiments were performed to determine the concentration of 6: 2 FTOH and its degradation products by LC-MS / MS.The bacterial community structure was analyzed by denaturing gradient gel electrophoresis and high-throughput sequencing.The results showed that 6: (6-FTCA), 6:2 FTUCA intermediate and 5:2 FT Ketone, 5:2 s FTOH, PFHx A, PFPe A, PFBA, 5: 3 Acid And other stable products, the process of sediment bacterial community structure had a significant impact, causing bacterial community richness and diversity changes in the 6:2 FTOH degradation of different stages of bacterial changes and dominant bacteria slightly different.According to 100 The experimental results of d, from the classification of the door At the level of 6:2, biodegradation of FTOH led to a sharp rise in the abundance of C. dipsaci (+ 24.8%) and a substantial decrease in the abundance of proteobacteria and pectobacteria (-17.8% and -15.9%). At the horizontal level, 6: 2 FTOH biodegradation caused the Anadromycinae (+ 19.6%) and the δ-Proteobacteria (+ 4.3%), which caused a large increase in abundance, causing ε-deformation (-20.0%), Clostridia (-10.1%), Bacillus (-5.8%) and Gamma proteobacteria (-4.2%) .According to the classification level of 6:2 FTOH, it is caused by biodegradation Vibrio (-14.1%), Sulfomonas (-13.2%) with larger decrease in abundance were found in Anaerolineaceae_uncultured (+ 19.1%) and Sulfurococcus (+ 13.3% ), Bacillus (-5.1%), Sulfurovum (-4.2%) and Fusibacter (-4.1%). These results help predict the response of bacteria to polyfluoroalkyls in the environment and screen biodegradable polyfluoroalkanes Bases of bacteria.