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壳寡糖可以增强植物对病虫害的防御能力,为了深入研究壳寡糖的作用机理,首次运用荧光酶标仪及一氧化氮(Nitric oxide,NO)荧光探针Diaminofluorescein diacetate(DAF-2DA)对壳寡糖诱导的NO信号进行研究。研究发现,不同浓度的壳寡糖均可诱导烟草悬浮细胞产生NO;NO的清除剂Carboxy-PTIO potassium salt(cPTIO)和一氧化氮合酶(Nitric oxide synthase,NOS)抑制剂Nω-nitro-L-arginine methyl Ester(L-NAME)可以明显抑制NO的产生;硝酸还原酶(Nitratereductase,NR)的抑制剂叠氮化钠和钨酸钠对NO的产生无影响;Ca2+流相关抑制剂氯化镧和钌红均可抑制NO的产生。NO和Ca2+流的相关抑制剂可明显抑制壳寡糖诱导的抗性相关基因的表达。结果显示:壳寡糖主要通过NOS酶催化合成NO,且NO参与调节壳寡糖诱导的抗性相关基因的表达,在此过程中,Ca2+可以调节NO的合成。
Chitooligosaccharides can enhance plant defense against pests and diseases. In order to further study the mechanism of chitooligosaccharides, chitooligosaccharides (DAF-2DA) and fluorescent probe Diaminofluorescein diacetate (DAF-2DA) Oligosaccharides induced NO signal was studied. The study found that different concentrations of chitooligosaccharides can induce NO production in tobacco suspended cells; NO scavenger Carboxy-PTIO potassium salt (cPTIO) and nitric oxide synthase (NOS) inhibitor Nω-nitro-L -arginine methyl Ester (L-NAME) could significantly inhibit the production of NO. Nitrate reductase (NR) inhibitor sodium azide and sodium tungstate had no effect on the production of NO. Ca2 + -labeled inhibitor lanthanum chloride And ruthenium red can inhibit the production of NO. Inhibitors of NO and Ca2 + currents significantly inhibited the expression of chitooligosaccharides induced resistance-related genes. The results showed that the chitooligosaccharides mainly catalyzed the synthesis of NO by NOS, and NO was involved in the regulation of chitooligosaccharides-induced resistance-related gene expression. During this process, Ca2 + could regulate NO synthesis.