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以陆地棉为母本分别与海岛棉、亚洲棉杂交，以海岛棉为母本与亚洲棉杂交，取杂交授粉后２、４、５、８天的受精胚珠进行离体培养，研究培养其杂种胚珠的适宜时期和适宜培养条件。结果表明，３个杂交组合均获得一定频率的胚；以陆地棉为母本的杂种胚珠获得了成熟胚及成熟胚萌发的苗；进行杂种胚珠培养的适宜时期为授粉后４～６天；同一组合的杂种胚珠，在不同培养基上获得的胚和成熟胚的频率不同；单一的培养基不能满足杂种胚珠的培养需要；产生胚和胚萌发的频率不仅与培养条件有关，与其杂交组合的亲本的生理特性也有很大关系。
Gossypium hirsutum L. was crossed with sea-island cotton and cotton as the female parent, and the island cotton was used as the female parent to cross with Asian cotton. The fertilized ovules at 2, 4, 5 and 8 days after hybridization pollination were cultured in vitro, Ovule proper period and suitable culture conditions. The results showed that all the three crosses obtained the embryos with a certain frequency. The germination of the mature and mature embryos was achieved by the hybrid ovules of G. hirsutum. The suitable period of hybrid ovule culture was 4 ~ 6 days after pollination. The frequency of hybrid ovules and hybrid embryos obtained on different media is different. The single culture medium can not meet the needs of hybrid ovules. The frequency of germination of embryos and embryos is not only related to the culture conditions, but also to the parents of hybrid combinations The physiological characteristics are also very much related.