论文部分内容阅读
目的:观察益气血补肝肾方对控制性超促排卵小鼠子宫内膜组织中整合素β3表达的影响。方法:将实验小鼠随机分为COH模型组、中药治疗组和对照组。进入实验后,中药治疗组每日给予中药灌胃,其余小鼠以等量生理盐水代替。COH模型组和中药治疗组给予GnRHa降调节,PMSG+hCG促排。每组分别于孕第1日(pd1)、pd2、pd4、pd5、pd6处死10只动物,取子宫内膜采用实时荧光定量RT-PCR和免疫印迹的方法分别在mRNA和蛋白水平定量检测子宫内膜整合素β3表达情况。于妊娠第8日(pd 8)剖腹取各组小鼠子宫(每组10只),观察胚泡着床情况。结果:整合素β3在各组小鼠子宫内膜中呈周期性的表达,从孕第1日(pd 1)开始整合素β3表达逐渐增强,pd 4显著增加,pd 6表达开始减弱。与COH模型组相比,中药治疗组小鼠子宫内膜中整合素β3表达增强,两者相比有统计学意义(P<0.05);整合素β3在中药治疗组和对照组中的表达水平相近,两者相比无统计学意义(P>0.05)。COH模型组pd 8的平均胚胎着床数与中药治疗组和对照组相比,明显减少(P<0.05),而中药治疗组与对照组相比无统计学差异(P>0.05)。结论:益气血补肝肾方可诱导小鼠子宫内膜中整合素β3的表达,并提高超促排卵小鼠的胚胎着床率,提示该方可能具有改善子宫内膜容受性的作用。
Objective: To observe the effect of Yiqi Xue Bugan Shendang on the expression of integrin β3 in endometrial tissue of mice with controlled hyperstimulation. Methods: The experimental mice were randomly divided into COH model group, TCM treatment group and control group. Into the experiment, the traditional Chinese medicine treatment group given daily gavage, the remaining mice with an equal volume of saline instead. COH model group and traditional Chinese medicine treatment group GnRHa down regulation, PMSG + hCG promote row. Each group was sacrificed on the first day of pregnancy (pd1), pd2, pd4, pd5, pd6 killed 10 animals, take endometrial real-time quantitative RT-PCR and Western blot were used in quantitative detection of intrauterine mRNA and protein levels Membrane integrin β3 expression. On the 8th day of gestation (pd 8), the uterus of mice in each group (10 in each group) were taken by cesarean section to observe the implantation status of blastocyst. Results: Integrin β3 expressed cyclically in the endometrium of mice. The expression of integrin β3 increased gradually from the first day of pregnancy (pd 1), the level of pd 4 increased significantly and the expression of pd 6 began to decrease. Compared with COH model group, the expression of integrin β3 in the endometrium of Chinese medicine treatment group was increased, the difference was statistically significant (P <0.05); the expression level of integrin β3 in the Chinese medicine treatment group and the control group Similar, no significant difference between the two (P> 0.05). Compared with the traditional Chinese medicine treatment group and the control group, the mean embryo implantation number of pd8 in COH model group was significantly decreased (P <0.05), while there was no significant difference between the traditional Chinese medicine treatment group and the control group (P> 0.05). Conclusion: Yiqi Xue Bugan decoction can induce the expression of integrin β3 in mouse endometrium and improve the embryo implantation rate in hyperstimulant mice, suggesting that this prescription may have the effect of improving endometrial receptivity. .