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目的:对使用改良高脂饲料建立稳定的高脂血症动物模型进行评价。方法:将80只SD大鼠随机分成对照组、普通高脂饲料组(普通组)、脂肪乳组和改良高脂饲料组(改良组),每组20只,雌雄各半;对照组给予普通饲料喂养,普通组、改良组、脂肪乳组分别给予普通高脂饲料、改良高脂饲料、脂肪乳喂养,制备高脂血症模型。于造模第11天、第31天分别采血检测大鼠血脂4项指标;观察比较第31天各组肝脏组织病理变化。结果:与对照组比较,脂肪乳组、普通组和改良组SD大鼠造模第11天、第31天血脂4项指标结果显示TC、TG、LDL-C升高(P<0.01或P<0.05),HDL-C差异无统计学意义(P>0.05);改良组TC、TG、LDL-C较普通组升高,差异有统计学意义(P<0.01或P<0.05),与脂肪乳组比较差异无统计学意义(P>0.05)。3模型组肝细胞均出现了脂肪样变性。结论:采用改良高脂饲料喂养SD大鼠,能解决大鼠厌食和死亡的问题、建立稳定的高脂血症大鼠模型。
OBJECTIVE: To evaluate the establishment of a stable animal model of hyperlipidemia using modified high-fat diet. Methods: Eighty SD rats were randomly divided into control group, normal high fat diet group (normal group), fat emulsion group and modified high fat diet group (modified group) Feed, normal group, modified group, fat emulsion group were given ordinary high-fat diet, improved high-fat diet, fat emulsion feeding, preparation of hyperlipidemia model. On the 11th day and the 31st day of modeling, four indexes of blood lipid in rats were determined by blood sampling. The pathological changes of liver tissues in each group were observed and compared on the 31st day. Results: Compared with the control group, the lipid, the normal group and the modified SD rats on the 11th and the 31st day showed higher levels of TC, TG and LDL-C (P <0.01 or P < (P <0.01 or P <0.05). The levels of TC, TG and LDL-C in the modified group were significantly higher than those in the normal group (P <0.01 or P <0.05) There was no significant difference between the two groups (P> 0.05). 3 model group of fatty liver degeneration have emerged. Conclusion: The feeding of SD rats with modified high-fat diet can solve the problem of anorexia and death in rats and establish a stable rat model of hyperlipidemia.