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以光敏核不育水稻农垦５８ｓ及其原始株农垦５８核ＤＮＡ为模板，进行了ＲＡＰＤ和ＩＳＳＲ多态性分析，结果表明：在可扩增的 １５４个ＲＡＰＤ引物和７８个 ＩＳＳＲ引物中， ８个ＲＡＰＤ引物和７个 ＩＳＳＲ引物的扩增产物表现出 ＤＮＡ多态性，ＲＡＰＤ－ＰＣＲ产物的多态性标记分别出现在农垦５８ｓ，农垦５８或二者同时出现，而ＩＳＳＲ－ＰＣＲ扩增产物的多态性标记只出现在农垦５８或农垦５８ｓ中。获得多态性ＲＡＰＤ标记及ＩＳＳＲ标记分别为２３个和１３个，其中１０个ＲＡＰＤ扩增片段和７个ＩＳＳＲ扩增片段为农垦５８ｓ所特有。本研究对ＲＡＰＤ－ＰＣＲ和ＩＳＳＲ－ＰＣＲ扩增产物采用非变性的聚丙烯酰胺凝胶电泳，结合快速银染法染色，与琼脂糖胶电泳，ＥＢ染色相比，大大提高了分辨率，值得推广应用。本文还对获得的ＭＰＤ及ＩＳＳＲ多态性片段进一步通过ＢＳＡ分析，或用转育前后的ｍＲＮＡ作Ｎｏｒｔｈｅｒｎ杂交分析加以鉴定等问题作了探讨。
The results of RAPD and ISSR polymorphism analysis indicated that among 154 amplified RAPD primers and 78 ISSR primers, 8 of Nanshan 58s and 58 of Nanshan 58 were used as template. The amplified products of RAPD primers and 7 ISSR primers showed DNA polymorphism. The polymorphic markers of RAPD-PCR products appeared at the same time of Nongken 58s and Nongken 58, respectively. However, the amplified products of ISSR-PCR The morphological markers only appear in Nongken 58 or Nongken 58s. The polymorphic RAPD markers and ISSR markers were 23 and 13, respectively. Among them, 10 RAPD amplified fragments and 7 ISSR amplified fragments were unique to Nongken 58s. In this study, non-denaturing polyacrylamide gel electrophoresis (PAGE) was used to amplify RAPD-PCR and ISSR-PCR amplification products, combined with rapid silver staining. Compared with agarose gel electrophoresis and EB staining, this method greatly enhanced the resolution and was worth promoting application. In addition, the obtained MPD and ISSR polymorphism fragments were further analyzed by BSA or by using Northern blot hybridization analysis of mRNAs before and after transfection.