论文部分内容阅读
目的:探讨Let-7c在乳腺浸润性导管癌中的表达情况及与各种临床病理特征的关系。方法:采用茎环RT-PCR方法检测40例乳腺浸润性导管癌及其对应的正常乳腺组织中Let-7c的表达,分析Let-7c表达与临床分期、淋巴结转移、增殖指数等临床病理特征的关系。结果:用茎环RT-PCR方法检测Let-7c表达的敏感性和特异性良好;Let-7c在乳腺癌组织中的表达显著低于正常组织(P<0.05);Let-7c的表达与乳腺癌的增殖指数有关(P<0.05),与雌孕激素受体状态亦有关,与是否绝经、乳腺癌的TNM分期、淋巴结转移未见显著统计学意义(P>0.05)。在高增殖指数、雌孕激素受体阴性的肿瘤组Let-7c表达明显降低。结论:Let-7c在乳腺浸润性导管癌中的表达异常,可能和乳腺癌的发生和发展有关,有望成为乳腺癌新的预后指标及治疗靶点。
Objective: To investigate the expression of Let-7c in breast invasive ductal carcinoma and its relationship with various clinicopathological features. Methods: The expression of Let-7c in 40 cases of breast invasive ductal carcinoma and its corresponding normal breast tissues was detected by stem-loop RT-PCR. The expressions of Let-7c, clinical stage, lymph node metastasis and proliferation index were analyzed relationship. Results: The sensitivity and specificity of Let-7c expression detected by stem-loop RT-PCR method were good. The expression of Let-7c in breast cancer tissues was significantly lower than that in normal tissues (P <0.05) (P <0.05), but also with the status of estrogen and progesterone receptors. There was no significant difference between menopausal women, TNM stage and lymph node metastasis of breast cancer (P> 0.05). In high proliferative index, the expression of Let-7c in estrogen and progesterone receptor-negative tumors was significantly lower. Conclusion: The abnormal expression of Let-7c in invasive ductal carcinoma of the breast may be related to the occurrence and development of breast cancer, which is expected to become a new prognostic indicator and therapeutic target for breast cancer.