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目的 :观察不同病因如黄曲霉毒素 B1 ( AFB1 )和 (或 )人乙型肝炎病毒 ( HBV )诱发树肝细胞癌 ( HCC)形成过程中 ,胰岛素样生长因子 ( IGF- )的动态变化。方法 :成年树按不同处理分为 A ( HBV+AFB1 )、 B ( HBV)、 C( AFB1 )、D (空白对照 ) 4组。各组动物定期肝活检 ;存活动物于实验 160周时处死。各次活检以及处死的动物肝组织均用免疫组化 S- P法进行 IGF- 蛋白表达的检测 ,并辅以常规病理组织学及 HBs Ag等 HBV感染标志的检查。结果 :HCC的发生仅见于 A、 C组 ,分别为 66.7% ( 14 / 2 1)和 3 0 .0 % ( 3 / 10 ) ,该两组的 IGF- 阳性率曲线亦高于其余两组 ,其中 C组的曲线虽曾在实验中期高于 A组 ,但不稳定 ;A组则于实验期间一直较稳定地保持在较高水平。B组仅有大增生结节但无 HCC发生 ,该组的 IGF- 阳性表达率曲线在 3个实验组中最低 ,仅高于空白对照 D组。结论 :IGF- 在 HCC的形成过程中呈较稳定、高水平的表达 ,但这种现象与 HCC的诱发因素为化学致癌物还是病毒致癌因素无关
OBJECTIVES: To observe the dynamic changes of insulin-like growth factor (IGF-I) during hepatocellular carcinoma (HCC) formation induced by different etiologies such as aflatoxin B1 (AFB1) and (or) human hepatitis B virus (HBV). Methods: Adult tree shrews were divided into four groups: A (HBV+AFB1), B (HBV), C (AFB1), and D (blank control). Animals from each group were subjected to regular liver biopsy; surviving animals were sacrificed at 160 weeks of experimentation. Each liver biopsy and sacrificed animal liver tissue were detected by immunohistochemical S-P IGF-protein expression, supplemented by routine histopathology and HBsAg and other markers of HBV infection. Results: The incidence of HCC was only found in group A and group C, which was 66.7% (14/21) and 30% (3/10) respectively. The IGF-positive rate curves of the two groups were also higher than the other two groups. Although the curve in the C group was higher than the A group in the middle of the experiment, it was not stable; the A group remained stable at a relatively high level during the experiment. In group B, there were only large nodular nodules but no HCC occurred. The IGF-positive expression rate curve in this group was the lowest in the three experimental groups and only higher than the blank control D group. Conclusion :IGF- is a stable and high-level expression during the formation of HCC, but this phenomenon is not related to whether HCC is induced by chemical carcinogens or viral carcinogens.