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目的从兴奋性氨基酸及代谢组学的角度,探讨芎芪合剂抗溶栓后脑缺血再灌注损伤的整体作用机理。方法建立SD大鼠大脑中动脉闭塞(MCAO)模型,模拟溶栓成功后的再灌注。分为假手术组、模型组、尿激酶组、芎芪合剂组、尿激酶加芎芪合剂组,每组30只。运用组织生化及核磁共振(NMR)代谢组学的方法,测定兴奋性氨基酸含量和血浆代谢组学相关指标。结果芎芪合剂能够抑制MCAO大鼠缺血再灌注引起的缺血侧脑组织兴奋性氨基酸谷氨酸(Glu)、天冬氨酸(Asp)含量的增高(P<0.05),并使之趋向于正常;代谢组学检测结果显示,芎芪合剂能抑制MCAO大鼠缺血再灌注引起的血浆谷氨酸以及乳酸、肌酸、胆碱代谢水平的升高和苏氨酸、丙氨酸以及血糖代谢水平的减低(P<0.05)。结论芎芪合剂能够调节大鼠缺血再灌注后的代谢平衡,从而起到抗脑缺血再灌注损伤的作用。
Objective To investigate the mechanism of action of Xiongqi Mixture after cerebral thrombolysis and cerebral ischemia-reperfusion injury from the perspective of excitatory amino acids and metabolomics. Methods The middle cerebral artery occlusion (MCAO) model of SD rats was established and the reperfusion after successful thrombolysis was simulated. Divided into sham operation group, model group, urokinase group, Xiongqi mixture group, urokinase and Xiongqi mixture group, 30 rats in each group. The histochemical and nuclear magnetic resonance (NMR) metabolomics methods were used to determine the excitatory amino acid content and plasma metabolomics related indexes. Results Xiongqi Mixture can inhibit the increase of excitatory amino acids glutamic acid (Glu) and aspartic acid (Asp) in ischemic brain tissue induced by ischemia reperfusion in MCAO rats (P <0.05) The results of metabonomics test showed that Xiong Qi Mixture can inhibit the increase of plasma glutamate, lactate, creatine and choline metabolism and the increase of threonine, alanine and Blood glucose levels were reduced (P <0.05). Conclusion Xiongqi Mixture can regulate the metabolic balance after ischemia-reperfusion in rats and thus play a role in anti-cerebral ischemia-reperfusion injury.