建立了柱后衍生-高效液相色谱法分离检测烟草中19种游离氨基酸的方法。样品经0.005 mol/L的盐酸超声提取,高效锂阳离子交换柱分离后,采用OPA柱后衍生,荧光检测器检测。各氨基酸在样品含量范围内呈良好的线性关系,相关系数(R2)均大于0.999,在烤烟中的加标回收率为87.73%～100.02%,相对标准偏差在2.7%～6.0%之间,检出限0.13～2.00μmol/L。方法可用于烟草中游离氨基酸的分析。 A post-column derivatization-high performance liquid chromatography (HPLC) method for the determination of 19 free amino acids in tobacco was established. Samples were extracted by 0.005 mol / L hydrochloric acid and separated by high efficiency lithium cation exchange column. The samples were detected by fluorescence detector after OPA column derivatization. The correlation coefficients (R2) were greater than 0.999 for each amino acid in the sample range, the recoveries were in the range of 87.73% -100.02%, and the relative standard deviations were between 2.7% and 6.0% The limits of 0.13 ~ 2.00μmol / L. The method can be used for the analysis of free amino acids in tobacco.