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以福鼎大白无性系茶树大田种子胚为材料，切取子叶、胚芽、胚轴和胚根进行培养，诱导形成愈伤组织。选用较致密的愈伤组织切块与带ＮＰＴⅡ和ＧＵＳ基因Ｔｉ质粒的根癌农杆菌共培养，通过卡那霉素抗性选择，得到了抗性愈伤组织并得到了芽的分化。抗性愈伤组织的诱导率在５％左右。共培过程中发现茶树愈伤组织能较强烈地促进农杆菌生长，表现在农杆菌在茶树愈伤组织周围的聚结。这可能是由于茶树多酚类含量较高，而通常酚基复合物有利于农杆菌对植物细胞的附着和转化。
Fuding Dapai clones tea field seed embryo as material, cut cotyledon, germ, hypocotyl and radicle for culture, inducing the formation of callus. The more compact callus cuttings were co-cultured with Agrobacterium tumefaciens with NPTⅡ and GUS gene Ti plasmids. Through selection of kanamycin resistance, resistant callus was obtained and bud differentiation was obtained. The induction rate of resistant callus is about 5%. During the process of co-culture, it was found that the callus of tea tree could strongly promote the growth of Agrobacterium, which showed the coalescence of Agrobacterium tumefaciens around the callus of tea plant. This may be due to the higher content of polyphenols in tea plants, whereas the phenol-based complexes generally favor the attachment and transformation of Agrobacterium to plant cells.