In this paper, we extend the generalized multiresolution analysis (GMRA)to higher dimensional spaces. The GMRA is generalized from each ladder space expanded by a different scaling function with positive integer dilation factor m ≥ 2.The n-d GMRA is discussed in orthogonal and bi-orthogonal cases. Then the optimal m-band wavelets are applied in processing the image datasets of the human body slices. The efficiency and superiority of the algorithm can be seen from the processing results.