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目的测定丽水市一起学校病毒性胃肠炎暴发疫情诺如病毒的RNA聚合酶区(RdRp)和衣壳蛋白区(VP1)基因序列,从分子水平对病原进行分型鉴定。方法收集疫情现场现症患者肛拭子、呕吐物、各类饮用水、可疑食物留样标本总计98份,采用荧光定量PCR方法检测诺如病毒,将阳性标本通过测序引物RT-PCR扩增RdRp和VP1基因序列并测定,通过生物信息学软件进行基因分型鉴定和序列分析。结果共检出诺如病毒阳性4份,阳性检出率为4.08%(4/98),均为GⅡ基因型;RdRp和VP1序列BLAST和种系发生分型结果均显示为诺如病毒GⅡ.4亚型。结论诺如病毒优势流行株GⅡ.4亚型是本次学校病毒性胃肠炎暴发疫情的重要病原体。
Objective To determine the RNA polymerase region (RdRp) and the capsid protein region (VP1) gene sequence of norovirus in a school with viral gastroenteritis outbreak in Lishui City and to identify the pathogens from the molecular level. Methods A total of 98 samples of anal swab, vomit, drinking water and suspicious food samples were collected from patients who were present on the scene of epidemic situation. Norovirus was detected by real-time fluorescence quantitative PCR, and positive samples were amplified by RT-PCR with primers RdRp And VP1 gene sequence were determined and genotyping identification and sequence analysis by bioinformatics software. Results A total of 4 norovirus positive isolates were detected, with a positive rate of 4.08% (4/98). All of them were GⅡ genotypes. The genotyping results of RdRp and VP1 sequences showed that they were norovirus G Ⅱ. 4 subtype. Conclusion The predominant epidemic strain of Norovirus, GⅡ.4, is an important pathogen of the outbreak of viral gastroenteritis in this school.