密穗高粱总DNA导入水稻的研究

来源 :湖南农业大学学报 | 被引量 : 0次 | 上传用户:ode
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为了选育高产、优质、多抗的水稻新品种,采用花粉管通道法,将密穗高粱总DNA导入水稻晚粳品种鄂宜105,从D1至D5代变异材料中选育出DH3,DH4,DH5等多个具有高光效、高产和优质的新品系.经随机扩增多态DNA技术对供体、受体、后代进行分子验证,后代中出现了在供体中存在而在受体中不存在的泳带.解剖学观察发现了后代剑叶、穗颈及穗基的输导组织比受体发达,维管束数目比受体增加10%~30%.后代DH3,DH4的光合速率亦显著高于受体.在形态解剖、光合特性和分子水平上进一步证明了高粱DNA片段在受体中的表达和稳定遗传.对DH3,DH4,DH5等品系进行生产示范,增产效果显著,单产高达7500kg/hm2.DH5已于1999年2月通过长沙市品种审定,定名为长晚粳1号.米质分析结果表明,整精米率、蛋白质含量、支链淀粉等多项指标均优于部颁优质米标准 In order to breed new rice varieties with high yield, high quality and multi-resistance, the total DNA of rice sorghum was introduced into the rice japonica variety E-Yi 105 by pollen tube channel method. DH3, DH4, DH5 and many other high light efficiency, high yield and high quality new lines. The donor, recipient and offspring were molecularly verified by random amplified polymorphic DNA technology. In the offspring, there appeared a swim band that existed in the donor and did not exist in the recipient. Anatomical observation revealed that the transduced tissues of flag leaves, panicle neck and panicle of the offspring were more developed than the recipient, and the number of vascular bundles was increased by 10% -30% compared with that of the recipient. The photosynthetic rate of offspring DH3 and DH4 was also significantly higher than that of the recipient. Morphological anatomy, photosynthetic characteristics and molecular levels further proved sorghum DNA fragments in the receptor expression and stable inheritance. DH3, DH4, DH5 and other strains of production demonstration, a significant yield increase, yield as high as 7500kg / hm2. DH5 has passed the Changsha City Variety Approval in February 1999 and is named as Changyuejing No.1. The results of rice quality analysis showed that many indexes such as whole milled rice rate, protein content and amylopectin were better than the ministerial high quality rice standard
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