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目的探讨小肠黏膜边缘群细胞移植方法治疗大鼠短肠综合征的效果。方法选取胎龄为4周的Wistar小鼠作为小肠黏膜边缘群细胞的供体,并选取20只雌性大鼠作为肠黏膜边缘群细胞的受体大鼠,建立大鼠短肠综合征的模型,观察实验组的大鼠短肠综合征采取小肠黏膜边缘群细胞治疗的结果,并与对照组比较大鼠小肠的基本特征以及对营养物质的吸收情况。结果治疗后2组大鼠的体重呈现逐渐增加的趋势,且小肠黏膜边缘群细胞治疗后大鼠体重的增加程度明显高于对照组,差异具有统计学意义(P<0.05);其次,进行小肠黏膜边缘群细胞治疗的大鼠的小肠的湿度、长度以及面积均显著高于对照组,差异具有统计学意义(P<0.05);进行小肠黏膜边缘群细胞治疗的大鼠对木糖、脂肪的吸收率以及氮的利用率均显著高于对照组,差异具有统计学意义(P<0.05);行小肠黏膜边缘群细胞治疗的大鼠绒毛高度、隐窝高度分别为(0.41±0.09)mm、(0.21±0.03)mm,与对照组比较有显著的降低,而绒毛密度却明显要比对照组高,差异具有统计学意义(P<0.05)。结论使用小肠黏膜边缘群细胞移植的方法治疗大鼠短肠综合征能显著地提高小肠对营养的吸收能力,其在临床中应用具有一定的指导意义。
Objective To investigate the effect of small intestinal mucosal marginal group cell transplantation in the treatment of short bowel syndrome in rats. Methods Wistar mice with a gestational age of 4 weeks were selected as donors for the marginal group cells of small intestine mucosa. Twenty female rats were selected as recipients of intestinal mucosa marginal group cells to establish a model of short-bowel syndrome in rats. The experimental group of rats with short bowel syndrome to observe the results of treatment of small intestinal mucosal marginal group cells and compared with the control group, the basic characteristics of the small intestine and the absorption of nutrients. Results After treatment, the body weight of rats in two groups increased gradually, and the increase of body weight of the rats with small intestinal mucosa edge cells was significantly higher than that of the control group (P <0.05). Second, Mucosal marginal group cells in rats treated with small intestine humidity, length and area were significantly higher than the control group, the difference was statistically significant (P <0.05); small intestinal mucosal marginal group cell treatment of rats on xylose, fat Absorption rate and nitrogen utilization rate were significantly higher than those in the control group, the difference was statistically significant (P <0.05). The height of villus and crypt height of rats treated with small intestinal mucosal margin cells were (0.41 ± 0.09) mm, (0.21 ± 0.03) mm, which was significantly lower than that of the control group, but the density of villus was significantly higher than that of the control group, with statistical significance (P <0.05). Conclusion The treatment of short-bowel syndrome in rats by the transplantation of small intestinal mucosa fringe cells can significantly improve the intestinal absorption of nutrients, which is of clinical significance.