目的:研究南蛇藤Celastrus orbiculatus Thunb.叶降血糖有效部位的化学成分。方法:采用柱色谱和薄层色谱分离纯化化合物,用IR、MS、NMR等波谱技术鉴定结构。结果:分离并鉴定了7个化合物,分别为:山柰酚(1)、槲皮素(2)、山柰酚-7-O-α-L-鼠李糖苷(3)、山柰酚-3,7-二-O-α-L-鼠李糖苷(4)、槲皮素-3-O-β-D-葡萄糖苷(5)、杨梅苷(6)和山柰酚-3-O-芸香糖苷(7)。结论:首次报道南蛇藤叶降血糖有效部位的化学成分,且化合物5~7为首次从该植物中分离得到。 OBJECTIVE: To study the chemical constituents of the effective sites of hypoglycemia in Celastrus orbiculatus Thunb. Methods: The compounds were isolated and purified by column chromatography and thin layer chromatography. Their structures were identified by IR, MS and NMR. Results: Seven compounds were isolated and identified as kaempferol (1), quercetin (2), kaempferol-7-O-α-L-rhamnoside 3-O-α-L-rhamnoside (4), quercetin-3-O-β-D-glucoside (5), myrosin (6) and kaempferol- - rutinoside (7). Conclusion: The chemical constituents of the effective sites of hypoglycemic activity of S. sinensis were first reported, and compounds 5-7 were isolated from this plant for the first time.