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为研究桃根系与根结线虫互作的组织结构变化及抗病机理,对抗病种质‘白根甘肃桃1号’(Prunus kansuensis‘Baigen Gansu Tao 1’)和感病种质‘贝蕾’(P.persica‘Bailey’)实生幼苗进行室内人工接种南方根结线虫(Meloidogyne incognita),并对接种后6、12、18、24、30、36、42和48 h的根尖进行染色观察及接种后0、6、12、36、60和84 h的根尖组织结构变化进行石蜡切片观察。染色结果表明,根结线虫侵染‘白根甘肃桃1号’和‘贝蕾’的规律基本一致:在接种12 h后开始侵入,接种12~30h线虫数量逐渐增加,然后线虫沿维管束向上移动,根尖线虫数量趋于减少。但与‘贝蕾’相比,‘白根甘肃桃1号’的根结线虫侵染数量显著降低,在接种30 h时仅为‘贝蕾’线虫侵染数的28%。石蜡切片结果显示‘白根甘肃桃1号’根结构受破坏程度明显较低,侵入的线虫主要聚集在一定区域,且在侵染84 h时线虫聚集区域周围的细胞出现坏死反应。
In order to study the changes of tissue structure and resistance mechanism of peach root and root knot nematode, we studied the relationship between disease resistance, Prunus kansuensis’Baigen Gansu Tao 1 ’and susceptible germplasm’ Bei Lei ’ P.persica’Bailey ’) seedlings were inoculated with Meloidogyne incognita indoors, and the root tips at 6, 12, 18, 24, 30, 36, 42 and 48 h after inoculation were stained and observed The changes of apical organization at 0, 6, 12, 36, 60, and 84 h were observed by paraffin section. The staining results showed that the root-knot nematode infection ’white root Gansu peach 1’ and ’Bei Lei’ basically the same law: 12 h after inoculation began to invade 12-30 hours after inoculation, the number of nematodes gradually increased, and then the worm along the vascular bundle upward move , The number of apical nematodes tends to decrease. However, compared with ’Beilei’, the number of root-knot nematode infection in ’Bai Gen Gansu Peach 1’ was significantly reduced, only 28% of the number of ’Bei Lei’ nematodes inoculated 30 h after inoculation. The results of paraffin section showed that the root structure of ’Bai Gen Gansu Peach No.1’ was significantly damaged, and the nematodes invaded mainly gathered in a certain area, and the cells around the nematode aggregation area appeared necrotic reaction 84 h after infection.