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A full-length c DNA library from the antenna of Chrysopa pallens( Rambur) was constructed based on switching mechanism at 5’ end of RNA transcript( SMART) system. The purified double-stranded c DNA was ligated to vector SMART2 IFD,and transformed into Escherichia coli DH5ɑ by electroporation. The library constructed for female and male reached high titers of 2. 1 × 10~6 and 1. 8 × 10~6 pfu / m L,respectively. PCR results showed that the inserts varied from 400 bp to 2 000 bp with the average size larger than 500 bp and the recombination rate over 93. 0%. Further studies on those genes and large scale sequencing of the library may be helpful in screening new olfactory related genes in C. pallens.
The full-length c DNA library from the antenna Chrysopa pallens (Rambur) was constructed based on switching mechanism at 5 ’end of RNA transcript (SMART) system. The purified double-stranded DNA was ligated to vector SMART2 IFD, and transformed into Escherichia coli DH5ɑ by electroporation. The library constructed for female and male reached high titers of 2. 1 × 10 ~ 6 and 1. 8 × 10 ~ 6 pfu / m L, respectively. PCR results showed that the inserts varied from 400 bp to 2 000 bp with the average size larger than 500 bp and the recombination rate over 93.0%. Further studies on these genes and large scale sequencing of the library may be helpful in screening new olfactory related genes in C. pallens.