本文采用微电子薄膜技术制作了具有双铂工作基底电极的传感器芯片。用Nafion膜修饰电极表面,并用化学交联法分别固定上葡萄糖氧化酶、半乳糖氧化酶,即制成可同时测定葡萄糖、半乳糖的多功能生物传感器。该多功能生物传感器对葡萄糖、半乳糖测定的范围分别为0～8.0mmol/L和0～6.0mmol/L,响应时间分别为30s和40s左右。适宜的pH范围为7.0～8.0。同时测定葡萄糖、半乳糖双组分时无交叉干扰。并且由于Nafion膜的阳离子选择透过性,基本上可消除Vc等电活性物质的干扰。 In this paper, a sensor chip with a bipolar work base electrode is fabricated using microelectronic film technology. The surface of the electrode was modified with Nafion membrane, and glucose oxidase and galactose oxidase were respectively immobilized by chemical cross-linking method to form a multifunctional biosensor capable of simultaneously measuring glucose and galactose. The multi-functional biosensor for glucose, galactose determination of the range of 0 ~ 8.0mmol / L and 0 ~ 6.0mmol / L, the response time was 30s and 40s. The suitable pH range is 7.0 ~ 8.0. Simultaneous determination of glucose, galactose two components without cross-interference. And due to the cation selective permeability of the Nafion membrane, interference with Vc and other electroactive substances can be substantially eliminated.