目的利用RNA干扰方法下调EB病毒潜伏膜蛋白1(latent membrane protein,LMP1)的表达,探讨LMP1对化疗药物敏感性的影响。方法采用小分子干扰片段转染EB病毒阳性淋巴瘤B95.8细胞,RT-PCR和Western blot分别检测LMP1的mRNA和蛋白的表达水平;联合应用化疗药物顺铂,通过MTT法观察细胞增殖活性变化。结果干扰LMP1后,其mRNA和蛋白的表达均明显下调;细胞增殖能力减弱;细胞被阻滞在G0/G1期;并且细胞对顺铂的IC50明显降低。结论干扰LMP1提高了B95.8细胞对化疗药物的敏感性,揭示LMP1可能是一个潜在的药物靶点。 OBJECTIVE: To down-regulate the expression of latent membrane protein 1 (LMP1) by RNA interference and investigate the effect of LMP1 on chemosensitivity. Methods Small interfering RNA (shRNA) fragments were transfected into B95.8 cells, and the expression of LMP1 mRNA and protein was detected by RT-PCR and Western blot. The changes of cell proliferation were observed by MTT assay . Results The mRNA and protein expression of LMP1 was significantly down-regulated after LMP1 was knocked down; the cell proliferation was weakened; the cells were arrested in G0 / G1 phase; and the IC50 of cells to cisplatin was significantly decreased. Conclusion Interference with LMP1 increases the sensitivity of B95.8 cells to chemotherapeutic drugs and reveals that LMP1 may be a potential drug target.