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目的用PCR技术检测转人组织蛋白酶K基因鼠系,筛选携带目的基因的阳性小鼠。方法用PCR技术,对显微注射后出生的转人组织蛋白酶K基因小鼠、首建鼠的F1、F2、F3及F2代与野生型ICR小鼠的回交后代进行检测。结果共检测显微注射后出生的小鼠25只,阳性为1只,阳性率为4%;检测F1、F2、F3代小鼠分别为60、77和69只,阳性仔鼠数为35、56和63只,阳性率分别为550%、727%和913%;对回交后代进行检测,未见纯合的F2代小鼠。结论外源基因(人组织蛋白酶K基因)已经整合到小鼠的染色体上,并且按孟德尔遗传定律中的分离定律进行遗传。
Objective To detect the murine cathepsin K transgenic mice by PCR and screen the positive mice carrying the gene of interest. Methods The backcross progeny of wild-type ICR mice F1, F2, F3 and F2 of transgenic mice born after microinjection were detected by PCR. Results Twenty-five mice were born after microinjection, and the positive rate was 4%. The numbers of F1, F2 and F3 mice were 60, 77 and 69 respectively, the number of positive pups was 35, 56 and 63, respectively. The positive rates were 550%, 727% and 913%, respectively. Backcross progenies were tested and no homozygous F2 generation mice were found. Conclusion The exogenous gene (Human Cathepsin K gene) has been integrated into the mouse chromosome and inherited according to the separation law in Mendelian law of inheritance.