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目的 探讨蕈样肉芽肿(MF)皮损中树突细胞(DC)表型及其临床意义。方法 检测DC表面分子的单克隆抗体和免疫组化技术。结果 MF斑片/斑块期的表皮及真皮浅层内存在大量的未成熟DC和成熟DC,主要是CD1a+、CD1c+、Lag+/Langerin未成熟DC和CD83+/DC-Lamp+成熟DC。肿瘤期的真皮内也见大量的CD1a+、CD1c+未成熟DC和CD83+/DC-Lamp+成熟DC,但Lag+/Langerin+DC更多见于表皮和真皮浅层,真皮深层少见,而此处CD1a+、CD1c+未成熟DC明显增多。结论 在MF斑片/斑块期,表皮朗格汉斯细胞发生了迁移,可能参与了抗肿瘤免疫反应,而肿瘤期真皮内大量CD1a+DC可能对相应的免疫耐受产生作用。
Objective To investigate the dendritic cell (DC) phenotype in mycosis fungoides (MF) and its clinical significance. Methods Monoclonal antibodies against DC surface molecules and immunohistochemistry were detected. Results A large number of immature and mature DCs existed in the superficial layers of epidermis and dermis in the MF plaque / plaque stage, mainly CD1a +, CD1c +, Lag + / Langerin immature and CD83 + / DC-Lamp + mature DCs. Large numbers of CD1a +, CD1c + immature DCs and CD83 + / DC-Lamp + mature DCs were also seen in the dermis during the tumor stage, but Lag + / Langerin + DC was more common in the epidermis and the superficial dermis than in the deep dermis where CD1a +, CD1c + Mature DC significantly increased. Conclusions Epidermal Langerhans cells migrated during MF plaque / plaque phase, which may be involved in the anti-tumor immune response. However, a large number of CD1a + DC in the dermis during the tumor stage may have an effect on the corresponding immune tolerance.