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目的:建立小承气汤中肉桂酸、大黄酸、芦荟大黄素和川陈皮素的HPLC同时测定方法。方法:采用Apollo C18柱(150mm×4.6mm,5μm),流动相为乙腈和0.011%冰乙酸溶液,梯度洗脱,时间25min,流速1mL/min,检测波长为254nm。结果:肉桂酸、大黄酸、芦荟大黄素和川陈皮素的线性范围分别为0.08-0.47、0.58-2.02、0.19-0.37和0.02-1.39μg,精密度为0.44%、0.17%、0.52%和0.33%,平均回收率分别为97.3%、99.1%、99.3%和100.5%。结论:建立的方法简便、灵敏、准确,可用于小承气汤的质量控制。
Objective: To establish a method for simultaneous determination of cinnamic acid, rhein, aloe-emodin and nobiletin by Xiaochengqi Decoction. Methods: Apollo C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.011% glacial acetic acid. The gradient elution was 25 min with a flow rate of 1 mL / min. The detection wavelength was 254 nm. RESULTS: The linear ranges of cinnamic acid, rhein, aloe-emodin and nobiletin were 0.08-0.47,0.58-2.02,0.19-0.37 and 0.02-1.39μg with precision of 0.44%, 0.17%, 0.52% and 0.33 %, The average recovery was 97.3%, 99.1%, 99.3% and 100.5% respectively. Conclusion: The established method is simple, sensitive and accurate and can be used for the quality control of Xiao Cheng Qi Decoction.