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To date, some confusion exists in the literature conceing the employment of the diffusion driving force and the calculation of the diffusion velocity for the gas species in a two-temperature (2-T) plasma. In this letter,a derivation is presented conceing the species diffusion driving force and diffusion velocity under 2-T plasmaconditions. Expressions for the ambipolar diffusion coefficients and the electric conductivity of the 2-T plasmaare also given.