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目的探讨唾液乳杆菌对哮喘Balb/c小鼠气道高反应性及T-bet/GATA-3表达的影响。方法选择30只4周、体质量16~18 g、SPF级Balb/c雌性小鼠,随机分成3组:正常对照组(N)、哮喘组(A)、哮喘加唾液乳杆菌组(AH)。应用卵蛋白建立急性哮喘模型,无创肺功能仪测定小鼠气道反应性、病理HE染色观察肺部炎症改变、Real-time PCR法检测肺组织中GATA-3 m RNA及T-bet m RNA表达。结果哮喘组小鼠较正常对照组小鼠气道反应性增高,AH组较哮喘组小鼠气道反应性降低(P<0.05);病理HE染色见哮喘组支气管管壁增厚、管腔狭窄、气管及血管周围可见大量以嗜酸性粒细胞为主的炎症细胞浸润,管腔中较多炎性分泌物,AH组小鼠肺组织病理改变较哮喘组明显减轻;哮喘组小鼠肺组织中GATA-3m RNA表达水平较N组明显升高,T-betm RNA表达水平较N组明显降低(P<0.05);而AH组小鼠肺组织中GATA-3m RNA表达水平较哮喘组明显降低,T-betm RNA表达水平较哮喘组明显升高(P<0.05)。结论致敏前唾液乳杆菌灌胃一定程度上减轻了哮喘小鼠的气道高反应性及气道炎症,通过在转录水平增加T-betm RNA表达同时抑制GATA-3m RNA表达,进而改善哮喘小鼠Th1/Th2失衡,为支气管哮喘的免疫防治提供新的途径。
Objective To investigate the effects of Lactobacillus salivarius on airway hyperresponsiveness and T-bet / GATA-3 expression in asthmatic Balb / c mice. Methods Thirty SPB Balb / c female mice were randomly divided into three groups: normal control group (N), asthma group (A), asthma plus Lactobacillus salivarius group (AH) . Acute asthma model was established by using ovalbumin, the airway responsiveness was measured by noninvasive pulmonary function test, pathological changes of lung inflammation were observed by HE staining, the expression of GATA-3 mRNA and T-bet m RNA in lung tissue were detected by Real-time PCR . Results The airway responsiveness of asthmatic mice was higher than that of normal mice, and the airway responsiveness of mice in AH group was lower than that of asthmatic mice (P <0.05). The histopathological HE staining showed bronchial wall thickening and stenosis , A large number of inflammatory cells infiltrated by eosinophils were found around the trachea and blood vessels, and more inflammatory secretions were found in the lumen. The pathological changes in the lung tissue of AH mice were significantly reduced compared with the asthma group. In the asthma group, The mRNA expression of GATA-3m was significantly higher than that of N group and the expression of T-bet mRNA was significantly lower than that of N group (P <0.05), while the expression of GATA-3mRNA in lung tissue of AH group was significantly lower than that of asthma group T-betm RNA expression was significantly higher than the asthma group (P <0.05). Conclusion Lactobacillus salivarius prior to sensitization reduced the airway hyperresponsiveness and airway inflammation in asthmatic mice to a certain extent, and improved the expression of GATA-3m RNA by increasing the expression of T-bet mRNA at the transcriptional level Mouse imbalance of Th1 / Th2, for the prevention and treatment of bronchial asthma provide a new way.