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目的探讨分子烙印聚合物(molecularly imprinted polymer,MIP)分子识别能力的机制及影响因素。方法以抗胆碱能药物盐酸新托品为模板分子、甲基丙烯酸为功能单体、三羟甲基丙烷三甲基丙烯酸酯为交联剂在乙腈中合成MIP,采用固相萃取-高效液相色谱方法考察MIP对乙腈溶液中的模板分子和结构类似物盐酸苯环壬酯、盐酸戊乙奎醚及结构有一定差异的化合物樟柳碱和氯苯那敏的固相吸附行为。结果MIP的分子识别能力与其合成条件和实验条件密切相关。结论MIP对待测物的非特异性吸附主要由氢键作用引起;MIP的特异性识别过程中,识别位点与待测物的空间结构匹配起着更为重要的作用。
Objective To investigate the molecular recognition mechanism of molecularly imprinted polymer (MIP) and its influencing factors. Methods The anticholinergic drug hydrochloride was used as template molecule, methacrylic acid was used as functional monomer and trimethylolpropane trimethacrylate as crosslinking agent to synthesize MIP in acetonitrile. The solid phase extraction - high performance liquid Phase chromatographic method was used to investigate the solid-phase adsorption behavior of MIP on the template molecules and the structural analogues of phenynonate, penehyclidine hydrochloride and their structurally different compounds, anisodine and chlorpheniramine in acetonitrile solution. Results The molecular recognition ability of MIP is closely related to its synthesis conditions and experimental conditions. Conclusions The nonspecific adsorption of MIP to the analyte is mainly caused by hydrogen bonding. In the process of MIP specific recognition, the matching of the recognition site with the spatial structure of the analyte plays an even more important role.