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为提高外周血造血干细胞动员、采集、和移植后造血重建的效率，作者从１９９７ 年４ 月至１９９９ 年６月，进行了２２ 例异基因或自体外周血造血干细胞移植，对外周血造血干细胞动员、采集和移植后造血重建方案（ＨＸ－９７ 方案）作了系统观察。ＨＸ－９７方案的主要内容是：①外周血造血干细胞动员，采用ｒｈＧ－ＣＳＦ３００μｇ／天，皮下注射，共６ 天，第６ 剂在干细胞采集前９０分钟用； ②自体外周血造血干细胞动员采用大剂量化疗加造血刺激因子，化疗强度务必使外周血ＷＢＣ计数＜ １．０×１０９ ／Ｌ，待外周血ＷＢＣ计数从最低点刚显示回升时，开始使用ｒｈＧ－ＣＳＦ；③移植后恢复造血序贯使用ｒｈＧＭ－ＣＳＦ和ｒｈＧ－ＣＳＦ。造血干细胞动员后用ＣＯＢＥＳｐｅｃｔｒａ 血细胞分离机连续采集外周血单个核细胞。结果：２２ 例移植中１６ 例一次采集即成功，６ 例作了第二次采集。按受者体重计，采集后计数ＭＮＣ细胞数２．５～１０．７×１０８／ｋｇ；ＣＤ３４＋ 细胞２．５～２０．０×１０６／ｋｇ；ＣＤ３４＋ ＣＤ３３－ 早期造血干细胞１．８～７．５×１０６／ｋｇ；ＣＤ３４＋ ＣＤ３３＋ 晚期造血祖细胞０．７～１２．５×１０６ ／ｋｇ；ＣＦＵ－ＧＭ 集落３．５～６．３×
To improve the efficiency of peripheral blood hematopoietic stem cell mobilization, collection, and hematopoietic reconstitution after transplantation, the author performed 22 cases of allogeneic or autologous peripheral blood hematopoietic stem cell transplantation from April 1997 to June 1999 to mobilize peripheral blood hematopoietic stem cells. A systematic observation was made of the hematopoietic reconstitution protocol (HX-97 protocol). The main contents of the HX-97 program are: 1 Peripheral hematopoietic stem cell mobilization, using rhG-CSF 300 μg/day, subcutaneous injection for 6 days, and the 6th dose is used 90 minutes before stem cell collection; 2 mobilization of autologous peripheral blood stem cells is large Dosage chemotherapy plus hematopoietic stimulating factor, chemotherapy intensity must make the peripheral blood WBC count <1.0 × 109 / L, until the WBC count of peripheral blood just shows rise from the lowest point, began to use rhG-CSF; 3 restore hematopoietic sequence after transplantation Use rhGM-CSF and rhG-CSF. Peripheral blood mononuclear cells were continuously collected using a COBE Spectra blood cell separator after mobilization of hematopoietic stem cells. RESULTS: Twenty-two cases of 16 cases were successfully collected at one time, and 6 cases were collected for the second time. According to the weight of the recipient, the number of MNC cells counted after collection was 2.5-10.7×108/kg; CD34+ cells were 2.5-20.0×106/kg; CD34+CD33- early hematopoietic stem cells were 1.8-7. 5 × 106/kg; CD34+ CD33+ advanced hematopoietic progenitor cells 0.7～12.5×106/kg; CFU-GM colony 3.5～6.3×