由于药物对映体之间在药理、毒理及吸收等方面存在较大差异 ,因此 ,建立分离和测定对映体化合物的方法十分重要。本文综述HPLC法在分离和测定药物对映体的常用方法 ,包括手性衍生化试剂、手性流动相和手性固定相在药物对映体分离测定中的应用。对对映体化合物的分析鉴定有指导意义。 Due to the large differences in the pharmacological, toxicological and absorption aspects of drug enantiomers, it is important to establish a method for the separation and determination of enantiomeric compounds. This review summarizes the application of HPLC methods in the separation and determination of enantiomers of drugs, including chiral derivatisation reagents, chiral mobile phases and chiral stationary phases in the separation of enantiomers. Analysis of the enantiomeric compounds identification is instructive.